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antibodies against col1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antibodies against col1
    Antibodies Against Col1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 678 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/col1/COL1A1+XP+Rabbit+mAb/10__1016_slash_j__cej__2026__180563-145-27-30
    Average 98 stars, based on 678 article reviews
    antibodies against col1 - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway
    Article Snippet: Then, 30 μg of each protein sample was separated through 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Merck Millipore). .. After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IκBα (#2859; CST), IκBα (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C. .. After binding with secondary HRP-labelled Goat Anti-Rabbit IgG (H+L) (#A0208; Beyotime) or HRP-labelled Goat Anti-Mouse IgG (H+L) (#A0216; Beyotime) for 2 hours at room temperature, the protein bands were tested by the imaging system (Bio-Rad) with enhanced chemiluminescent ECL reagent (Biosharp).

    Incubation:

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway
    Article Snippet: Then, 30 μg of each protein sample was separated through 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Merck Millipore). .. After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IκBα (#2859; CST), IκBα (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C. .. After binding with secondary HRP-labelled Goat Anti-Rabbit IgG (H+L) (#A0208; Beyotime) or HRP-labelled Goat Anti-Mouse IgG (H+L) (#A0216; Beyotime) for 2 hours at room temperature, the protein bands were tested by the imaging system (Bio-Rad) with enhanced chemiluminescent ECL reagent (Biosharp).

    Fluorescence:

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Staining:

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Cell-Signaling:

    Article Title: Modafinil exerts anti-inflammatory and anti-fibrotic effects by upregulating adenosine A 2A and A 2B receptors
    Article Snippet: .. The membranes were immunoblotted using antibodies against A 1 AR (ab151523, Abcam), A 2A AR (ab3461, Abcam), A 2B AR (ab229671, Abcam) and A 3 AR (ab197350, Abcam), K Ca 3.1 (sc-365,265, Santa Cruz Biotechnology, Dallas, TX, USA), K Ca 2.3 (sc28621, Santa Cruz Biotechnology), Col1 (#66,948, Cell Signaling Technology Inc., Danvers, MA, USA), α-SMA (ab7817, Abcam), Epac1 (sc-28,366, Santa Cruz Biotechnology), and Epac2 (sc28326f, Santa Cruz Biotechnology), and bands were visualized by chemiluminescence. ..

    other:

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway.
    Article Snippet: Protein samples were denatured at 100°C for 5 min with sodium dodecyl sulphate polyacrylamide gel electrophoresis loading buffer (Beyotime).



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    LPS triggered an inflammatory response and suppressed the osteogenic differentiation potential of PDLSCs. (A) The proliferative capacity of PDLSCs exposed to varying LPS concentrations over 2, 4, and 6 days was measured with CCK-8 assay. (B) The qRT-PCR analysis revealed elevated mRNA expression of inflammatory mediators IL-6, IL-8 , and IL-10 in PDLSCs following treatment with 10 µg/mL LPS. The qRT-PCR (C) and WB (D) analysis revealed significantly lower expression of osteogenic markers <t>(COL1</t> and RUNX2) in PDLSCs exposed to 10 µg/mL LPS. The ALP (E) and alizarin red (F) staining assays showed that 10 µg/mL LPS significantly inhibited the osteogenic differentiation capacity of PDLSCs compared to the control group. Data were presented as mean ± SD ( n = 3). *** P < .001, **** P < .0001.
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    Image Search Results


    LPS triggered an inflammatory response and suppressed the osteogenic differentiation potential of PDLSCs. (A) The proliferative capacity of PDLSCs exposed to varying LPS concentrations over 2, 4, and 6 days was measured with CCK-8 assay. (B) The qRT-PCR analysis revealed elevated mRNA expression of inflammatory mediators IL-6, IL-8 , and IL-10 in PDLSCs following treatment with 10 µg/mL LPS. The qRT-PCR (C) and WB (D) analysis revealed significantly lower expression of osteogenic markers (COL1 and RUNX2) in PDLSCs exposed to 10 µg/mL LPS. The ALP (E) and alizarin red (F) staining assays showed that 10 µg/mL LPS significantly inhibited the osteogenic differentiation capacity of PDLSCs compared to the control group. Data were presented as mean ± SD ( n = 3). *** P < .001, **** P < .0001.

    Journal: International Dental Journal

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway

    doi: 10.1016/j.identj.2026.109417

    Figure Lengend Snippet: LPS triggered an inflammatory response and suppressed the osteogenic differentiation potential of PDLSCs. (A) The proliferative capacity of PDLSCs exposed to varying LPS concentrations over 2, 4, and 6 days was measured with CCK-8 assay. (B) The qRT-PCR analysis revealed elevated mRNA expression of inflammatory mediators IL-6, IL-8 , and IL-10 in PDLSCs following treatment with 10 µg/mL LPS. The qRT-PCR (C) and WB (D) analysis revealed significantly lower expression of osteogenic markers (COL1 and RUNX2) in PDLSCs exposed to 10 µg/mL LPS. The ALP (E) and alizarin red (F) staining assays showed that 10 µg/mL LPS significantly inhibited the osteogenic differentiation capacity of PDLSCs compared to the control group. Data were presented as mean ± SD ( n = 3). *** P < .001, **** P < .0001.

    Article Snippet: After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IκBα (#2859; CST), IκBα (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C.

    Techniques: CCK-8 Assay, Quantitative RT-PCR, Expressing, Staining, Control

    NLRP12 overexpression reduced LPS-induced inflammatory response and reversed LPS-induced suppression of osteogenic differentiation in PDLSCs. (A and B) The qRT-PCR and WB data revealed that expression level of NLRP12 in oeNLRP12 group was increased significantly compared to oeNC group. (C) The qRT-PCR data revealed that mRNA expression of IL-6 and IL-8 was suppressed, while the mRNA expression of IL-10 was increased in oeNLRP12 + LPS group compared to oeNC + LPS group. (D) The expression of COL1 and RUNX2 was increased significantly detected by WB assays in oeNLRP12 + LPS group compared to oeNC + LPS group. (E) Representative pictures of ALP staining showed lower staining in oeNC + LPS group compared to oeNC group and higher staining in oeNLRP12 + LPS group compared to oeNC + LPS group (scale bar = 500 μm). (F) Representative pictures of alizarin red staining showed fewer mineralized nodules in oeNC + LPS group compared to oeNC group and more mineralized nodules in oeNLRP12 + LPS group compared to oeNC + LPS group (scale bar = 500 μm). oeNC: PDLSCs transfected via negative control lentiviral. oeNLRP12: PDLSCs transfected via lentiviral with overexpression- NLRP12 . oeNC + LPS: PDLSCs transfected via negative control lentiviral and subsequently cultured under 10 µg/mL LPS induction. oeNLRP12 + LPS: PDLSCs transfected via lentiviral with overexpression- NLRP12 and subsequently cultured under 10 µg/mL LPS induction. Data were presented as mean ± SD ( n = 3). ns, no significant difference, *** P < .001, **** P < .0001.

    Journal: International Dental Journal

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway

    doi: 10.1016/j.identj.2026.109417

    Figure Lengend Snippet: NLRP12 overexpression reduced LPS-induced inflammatory response and reversed LPS-induced suppression of osteogenic differentiation in PDLSCs. (A and B) The qRT-PCR and WB data revealed that expression level of NLRP12 in oeNLRP12 group was increased significantly compared to oeNC group. (C) The qRT-PCR data revealed that mRNA expression of IL-6 and IL-8 was suppressed, while the mRNA expression of IL-10 was increased in oeNLRP12 + LPS group compared to oeNC + LPS group. (D) The expression of COL1 and RUNX2 was increased significantly detected by WB assays in oeNLRP12 + LPS group compared to oeNC + LPS group. (E) Representative pictures of ALP staining showed lower staining in oeNC + LPS group compared to oeNC group and higher staining in oeNLRP12 + LPS group compared to oeNC + LPS group (scale bar = 500 μm). (F) Representative pictures of alizarin red staining showed fewer mineralized nodules in oeNC + LPS group compared to oeNC group and more mineralized nodules in oeNLRP12 + LPS group compared to oeNC + LPS group (scale bar = 500 μm). oeNC: PDLSCs transfected via negative control lentiviral. oeNLRP12: PDLSCs transfected via lentiviral with overexpression- NLRP12 . oeNC + LPS: PDLSCs transfected via negative control lentiviral and subsequently cultured under 10 µg/mL LPS induction. oeNLRP12 + LPS: PDLSCs transfected via lentiviral with overexpression- NLRP12 and subsequently cultured under 10 µg/mL LPS induction. Data were presented as mean ± SD ( n = 3). ns, no significant difference, *** P < .001, **** P < .0001.

    Article Snippet: After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IκBα (#2859; CST), IκBα (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C.

    Techniques: Over Expression, Quantitative RT-PCR, Expressing, Staining, Transfection, Negative Control, Cell Culture

    Overexpression of NLRP12 alleviated the inflammatory responses and osteogenic differentiation inhibition of PDLSCs by suppressing the NF-κB pathway. (A) WB results showing the changes of protein expression levels of p-p65, p65, p-IκBα, and IκBα in PDLSCs after NLRP12 overexpression. (B) WB results showing the changes of protein expression levels of p-p65 and p65 in PDLSCs overexpressing NLRP12 after PMA treatment. (C)The qRT-PCR results demonstrating the transcriptional expression levels of IL-6, IL-8 , and IL-10 in PDLSCs overexpressing NLRP12 after PMA treatment. (D)WB results demonstrating alterations in the protein expression levels of COL1 and RUNX2 in PDLSCs overexpressing NLRP12 after PMA treatment. (E) Representative pictures showing ALP staining (scale bar = 500 μm) (F) Representative pictures showing alizarin red staining (scale bar = 500 μm). oeNC: PDLSCs transfected via negative control lentiviral. oeNLRP12: PDLSCs transfected via lentiviral with overexpression- NLRP12 . oeNC + LPS: PDLSCs transfected via negative control lentiviral and subsequently cultured under 10 µg/mL LPS. oeNLRP12 + LPS: PDLSCs transfected via lentiviral with overexpression- NLRP12 and subsequently cultured under 10 µg/mL LPS. Data were presented as mean ± SD ( n = 3). ns, no significant difference, * P < .05, ** P < .01, *** P < .001, **** P < .0001.

    Journal: International Dental Journal

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway

    doi: 10.1016/j.identj.2026.109417

    Figure Lengend Snippet: Overexpression of NLRP12 alleviated the inflammatory responses and osteogenic differentiation inhibition of PDLSCs by suppressing the NF-κB pathway. (A) WB results showing the changes of protein expression levels of p-p65, p65, p-IκBα, and IκBα in PDLSCs after NLRP12 overexpression. (B) WB results showing the changes of protein expression levels of p-p65 and p65 in PDLSCs overexpressing NLRP12 after PMA treatment. (C)The qRT-PCR results demonstrating the transcriptional expression levels of IL-6, IL-8 , and IL-10 in PDLSCs overexpressing NLRP12 after PMA treatment. (D)WB results demonstrating alterations in the protein expression levels of COL1 and RUNX2 in PDLSCs overexpressing NLRP12 after PMA treatment. (E) Representative pictures showing ALP staining (scale bar = 500 μm) (F) Representative pictures showing alizarin red staining (scale bar = 500 μm). oeNC: PDLSCs transfected via negative control lentiviral. oeNLRP12: PDLSCs transfected via lentiviral with overexpression- NLRP12 . oeNC + LPS: PDLSCs transfected via negative control lentiviral and subsequently cultured under 10 µg/mL LPS. oeNLRP12 + LPS: PDLSCs transfected via lentiviral with overexpression- NLRP12 and subsequently cultured under 10 µg/mL LPS. Data were presented as mean ± SD ( n = 3). ns, no significant difference, * P < .05, ** P < .01, *** P < .001, **** P < .0001.

    Article Snippet: After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IκBα (#2859; CST), IκBα (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C.

    Techniques: Over Expression, Inhibition, Expressing, Quantitative RT-PCR, Staining, Transfection, Negative Control, Cell Culture

    Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .

    Journal: International Dental Journal

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway

    doi: 10.1016/j.identj.2026.109417

    Figure Lengend Snippet: Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .

    Article Snippet: After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IκBα (#2859; CST), IκBα (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C.

    Techniques: Over Expression, Micro-CT, Staining, Immunohistochemical staining, Expressing, Control, Immunohistochemistry